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Combined Hydatid Disease Antibody Rapid Test (310600)

Combined Hydatid Disease Antibody Rapid Test (310600)
  • Product Detials:Catalog No. Hyd-01 For Professional Use Only Summary Hydatid diseas is caused by Hydatid parasitizing human, and then the specific antibody against Hydatid arise in human body, hereby detecting the specific antibody against Hydatid can be the secondary diadynamic criteria. The test take the Hydatid cysticercus antigen as the solid phase antigen to detect specific antibody against Hydatid in human serum. Reagents and contents 1 8X Coated microtiter strips: Ready to use 2 1X Conjugate solution: Ready to use 3 1X Washing solution: Ready to use 4 1X Substrate A solution: Ready to use 5 1X Substrate B solution: Ready to use 6 1X Sample dilution: Ready to use 7 1X Stopping solution: Ready to use Warning: Stopping solution irritates eyes and skin. Keep out of the reach of children. Upon contact with the eyes, rinse thoroughly with water and consult a doctor. 8 1X Positive control: Ready to use 9 1X Negative control: Ready to use 10 1X Instruction sheet Test procedure 1 Adding samples and controls Leave well A1 for reagent blank. Pipette controls and samples as follows: Add two drops diluent into each well, then add 10 ul serum sample into each well separately, mix evenly. Synchronously add positive control and negative control each 100 ul into each one well without diluent and add two drops diluent as the blank. Incubate in dark at 37 degree centigrade for 30 minutes, then discard the liquid of the well, add 1 drop washing solution into each well and fill with distilled water promptly, incubate for 30 seconds, then discard the liquid, continue perform another 4 washing cycles as above, then discard the liquid, flap to dry with the absorbent paper. 2 Adding conjugate solution Dispense 2 drops into all wells and incubate at 37 degree centigrade for 15 minutes. Discard the contents of the wells and wash 5 times as described in step 2. 3 Adding substrates Dispense one drop of substrate A solution and B solution respectively, incubate at room temperature (37 degree centigrade ) for 10 minutes. Dispense one drop of stopping solution into all wells. Zero the ELISA microtiter plate reader using the reagent blank well A1. Measure the absorbance at 450 nm. Results 1 Observation with naked eyes before adding stopping solution Negative: No apparent blue appears in the sample wells. This indicates that no cysticercus antibody has been detected. Positive: Apparent blue appears in the sample wells. This indicates that the sample contains cysticercus antibody. Invalid: If no blue appears in the positive control well or apparent blue is observed in the negative control well, this indicates a possible error in performing the test. The test should be repeated using a new kit. 2 Judgment by microtiter plate reader Cut-off value = 2.1XA- (absorbance of negative control) Interpretation of results A450 (patient) ≥ cut-off: Anti- P. Westermanii antibody positive A450 (patient) < cut-off: Anti- P. Westermanii antibody negative Notes 1. Store at 2-8 degree centigrade, make the temperature of the kit return to room temperature before use. Screw down the cap after use, don't mix caps between diffirent bottles and don't mix components between diffirent kits 2. Fill wells with distilled water when washing, 30seconds each time, pour out the liquid. Prohibit to use tap water or any other water. 3. Recommand to judge results with instruments to improve comparability of testing. 4. It is best to incubate in water bath box if the instrument is available. More Product Features Trademark: COMBINED Model NO.: 310600 Standard: ISO9001:2000 Company: Shenzhen Combined Biotech Co., Ltd.
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Contact Info
Company： Shenzhen Combined Biotech Co.,Ltd
Area： China/Guangdong
Contact： Mr. LI Hailong
Tel： 86-755-26650513
Fax： 86-755-26650520
Web site：
Add： Unit 2d, Building 8th, Tian-An Industrial Park, Nanyou, Nanshan District, Shenzhen, Guangdong, China
Zip： 518054

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   Shenzhen Combined Biotech Co.,Ltd

     Founded in 2003, Shenzhen Combined Biotech Co., Ltd (BIOCBD) is a high-tech enterprise, aiming ..... more >>

Contact： Mr. LI Hailong 

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