T3 ELISA Test Kit

Product Detials:Assay principle The T3 EIA, a second antibody (goat anti-mouse IgG) is coated on a microtiter wells. A measured amount of patient Serum, a certain amount of mouse monoclonal Anti-T3 antibody, and a constant amount of T3 conjugated with horseradish peroxidase are added to the microtiter wells. During incubation, the mouse anti-T3 antibody is bound to the second antibody on the wells, T3 and the enzyme conjugated-T3 compete for the limited binding sites on the anti-T3 antibody. After a 60 minute incubation at the temperature 37, the wells are washed 5 times by water to remove unbound T3 conjugate. A solution of H2O2/TMB is then added and incubated for 20 minutes at room temperature, resulting in the development of a blue color. The color development is stopped with the addition of 1N HCl, and the absorbance is measured spectrophotometrically at 450 nm. The intensity of the color formed is proportional to the amount of enzyme present, and is inversely related to the amount of unlabeled T3 standards assayed in the same way. The concentration of T3 in the unknown sample is then calculated. 7-11 Trademark: JD Model NO.: JT003 Company: Jei Daniel Biotech Corp.

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Company：	Jei Daniel Biotech Corp.
Area：	China/Shandong
Contact：	Ms. Wu
Tel：	86-531-82373551
Fax：
Web site：
Add：	A201,1st Building,No69,Huayang Road, Jinan, Shandong, China
Zip：	250100

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Jei Daniel Biotech Corp.

Company Introduction JD BIOTECH Ltd. originated in Taipei, Taiwan, and has existed since 1995. ..... more >>

Contact： Ms. Wu

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